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The various substituted ortho phenylenediamine and some other substituted cinnamic acid mixture were prepared in 10 mL of ethylene glycol contained in an RBF. This content was subjected to heat at 200°C for six-fourteen minutes on an oil bath. The product was recrystallized using ethanol. For scheme 2 Substituted ortho-phenylenediamine with substituted benzaldehyde, 0.01 mol each in the 100mL round bottom flask add 3mL hydrogen peroxide, and ceric ammonium nitrate (0.001 mol). The above content was subjected to heat maintaining the temperature at 500C for nine-thirty minutes. Antifungal studies of newly synthesized compounds were carried out against different unicellular and filamentous fungi. A qualitative evaluation of antifungal activity was resolved with the help of the well diffusion method. All the synthesized compounds confirm the presence of characteristic common groups as well as individual groups in 1HNMR spectroscopy. 13CNMR spectra of all the synthesized compounds show characteristic hydrogen as well as individual hydrogen of chemical structure. In experimental data denotes mass spectroscopic data of synthesized compounds. Well diffusion assay was performed for synthesized benzimidazole derivatives for determining the antifungal activity of compounds against various unicellular as well as filamentous fungi. Compounds 09, 02, 20, 16, 01 were evaluated for antifungal activity. Many of the compounds showed antifungal activity comparable to that of Amphotericin B (IC50= 0.84 -0.97 μg/ml), with compound 09 being the most active (IC50 =0.84 μg/ml less potent than amphotericin B).
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