Anti-microbial Activity of 3 Different Species of Tulsi Found in Chhattisgarh against Alcaligenes Faecalis & Salmonella Enterica

Abstract

Tulsi (Ocimum spp.), an aromatic plant belongs to the family, Lamiaceae. Tulsi can be grouped to two broad categories, namely holy basil (Ocimum sanctum) and mediterranean basil (Ocimum basilicum). The present study was carried out to compare phytochemical and antimicrobial activity of three Tulsi species, namely Rama Tulsi (Ocimum sanctum), Krishna Tulsi (Ocimum tenuiflorum), and mediterranean basil (Ocimum basilicum). These tulsi species can be found in different agro-climatic zones of Chhattisgarh. For anti-microbial activity against bacteria different extractions were made out of all three species (Petroleum ether extract, Ester extract, methanol extract, chloroform extract and formaldehyde extract). Two methods for anti-microbial activity testing were done i.e. well diffusion and disc diffusion method. Results showed that among all species of tulsi and two bacterial strains, Ocimum tenuiflorum showed maximum zone of inhibition with petroleum ether, ester and methanol extracts.