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N-substituted-1-phenyl-5,6-dimethyl-1,5-dihydro-4H-pyrazolo [4,3-d]pyrimidine has been synthesized by 1-phenyl-5-amino-4,5-dihydro-1H-pyrazole-4-carbox-amide derivatives as novel series were reacted with ester ethylacetate and aromtised as the pyrimidine ring the carbonyl functional group of the molecules were further halogenated by chlorination with phosphorous oxy chloride and yielded 1-phenyl-4-chloro-5,6-dimethyl-4,5-dihydro-1H-pyrazolo[4,3-d]pyrimidine: which further aminated with alkyl and aromatic amines are substituted chlorines. Compounds prepared were diluted to a concentration 50µL and other dilutions were prepared in 96well plates in triplicate and final volume made up to 100µL using DMSO solvent. 50-100µL of tris-base solution with pH10.5 were added to all wells and shaked in an equipment for shaking called orbital shaker and incubated for a duration of 10 min to stabilise the dye bounded to a protein. Absorbance has been measured at 510nm at a micro plate to obtain observations. Cell % growth = Absorbance sample/ Absorbance negative control X 100 % Growth inhibition = 100 - % Cell growth.
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