SCREENING AND SYSTEMATIC EVALUATION OF ACTIVE PHYTOCHEMICAL CONSTITUENT OF CYANOBACTERIAL ISOLATES

Abstract

Cyanobacteria drive biogeochemical cycle and produces active metabolites viz., carbohydrates, proteins, lipids, alkaloids, flavonoid, phenols and saponins. These phytochemical metabolic constituents have played a crucial role to retain cellular structure, cell to cell communication, cellular defence, healthcare and household applications. The present work was done to screen out and systematic evaluation of active phytochemical constituent of Cyanobacteria isolated from rice field and pond. Two hundred samples were screened for cyanobacteria strains and Anabaena sp. (45%) and Cylindrospermum sp. (42%) observed to occupy maximum density in the samples. The maximum carbohydrate content (33.7 %) and protein (25.48±0.29) content was observed in Anabaena sp. while lipid (9.93 %) and amino acid content (4.82±0.14) in Geitlerinema sp. Anabaena sp., Cylindrospermum sp., Cyclotella sp. and Diatoma sp were found to retain most active fractions. The combination of Column chromatography, HPLC and FTIR were used to identification these active fractions. The active fractions i.e., α-cyclocitral, limonene, inositol and hexa-decanoic acid were identified. These active phytochemical constituent could further be explored for their utilization in healthcare, household and industrial applications.