Stability Indicating LC–MS/MS method for Lamivudine and Nevirapine

Abstract

The aim of the stability studies is to perform to meet the quality, safety and efficacy. This type of study is used to know the changes in physical, chemical or microbiological properties of the drug with respect to time. Based on our study, noticed that Lamivudine and Nevirapine were stable to thermal stress. Percentage stability of all the stock solutions met the acceptance criteria. Working Solution percentage Stability values for Lamivudine and Nevirapine at LQC & HQC levels were 104.35 & 99.73% and 100.82 & 99.49%, respectively. Working Solution percentage Stability values for Lamivudine 13C 15N2 and Nevirapine D5 at LQC & HQC levels were 99.66 & 99.85% and 101.41 & 101.93, respectively. The stability studies were determined by calculating the Low Quality Control and High Quality Control samples percentage nominal beside freshly pointed, prepared calibration curve standards and compared with freshly spiked and prepared comparison samples at Low Quality Control and High Quality Control level..