Comparative Study on Anticoagulant Activity of Different Parts of Achyranthes Aspera

Abstract

Achyranthes aspera is a perennial herb belonging to the family of Amaranthaceae (Hossain et al 2013). It is known as “Prickly chaff flower”.The plant was found to have antibacterial property against hospital origin gram positive bacteria (Singh et al 2019). Achyranthes aspera contain coumarin which having protease activity. Coumarin is a potent enzyme that naturally supports the body's ability to break down blood clots caused by the blood-clotting protein fibrin. The aim of this work was to potent clot lysis activity (anticoagulant activity) of different parts of Achyranthes aspera with different solvent to get a fruitful comparative output. Achyranthes aspera contain coumarin which having protease activity. Extracts prepared using acetone as solvent gave maximum clot lysis activity (48.3%) as compare to other. However extracts prepared by taking ethanol and methanol were gave approximately same clot lysis. Extracts prepared by taking water as solvent gave minimum clot lysis <10% in all sample of Achyranthes aspera. Heparin as a positive control for anti coagulant activity shows the maximum percentage of clot lysis that is 76.1% at 0.5ml and 46% at 0.1 ml which is near to clot lysis percent of leaf extract prepared by using acetone as a solvent.