HPLC analysis and determination of saccharides in selected fruit and vegetable juice

Abstract

High performance liquid chromatography (HPLC) method for the determination of main three sugars (glucose, sucrose and fructose) individually by chromatography to identify and quantify the amount of mentioned sugars. The determination of sugar is important for quality and assurance of fruits and vegetables. The present work reports the sugar analysis of extracted apple juice, apple pulped juice, carrot juice and cabbage juice using HPLC method, the sugars (glucose, sucrose and fructose) were separately analysed and quantified using different revers phase methods. The higher amount of sugars were observed in pulped apple juice but the cabbage juice was contain lesser amount of sucrose and the lesser amount of fructose was seen in carrot juice.