Exploitation of Aspergillus fumigatus secondary metabolites and silver nanoparticle coated crude extract against pathogenic bacteria isolated from surgical site wound infection

Abstract

Multi drug resistance is increasing day by day due to misuse of antibiotics. Fungi have the ability to produce potent metabolites. They have potential to combat bacterial pathogens. Synthesis of silver nanoparticle   (AgNPs) has gained significant importance in recent years due to its simple, nontoxic, less time consuming and cost-effective nature. This study was focused on biosynthesis of AgNPs from the extract of Aspergillus fumigatus applying different analytical technique such as UV-visible spectroscopy, X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) for characterization of AgNPs. The effect of synthesis AgNPs and crude extract noted against different bacterial pathogens. Maximum antibacterial activity were noticed against tested bacteria by both fungal crude extract and (AgNPs) Maximum antibacterial activity of Ethyl acetate crude extract at 50µl concentration (12mg/1ml DMSO) showed (17mm) zone of inhibition against E.coli. While minimum antibacterial activity of Ethyl crude extract at 50µl concentration was observed against S.typhi (16mm). Highest antibacterial activity of Ethyl acetate crude extract at 100µl was noted against E.coli which showed (21mm) zone of inhibition. While (24mm) zone of inhibition was observed against S.typhi at 100µl concentration Ethyl acetate crude extract and AgNPs. While (25mm) zone of inhibition was observed against E.coli at 100µl concentration Ethyl acetate crude extract and AgNPs respectively. Surface Plasmon Resonance (SPR) observed at 432 nm during UV-visible spectroscopy which confirmed the synthesis of AgNPs. The SEM micrograph demonstrated the spherical shape of AgNPs. FTIR analysis revealed the involvement of phenolic, carboxyl and hydroxyl groups in reduction of Ag⁺ ions to form AgNPs while stabilization components of AgNPs were amide linkage and amino acid. The XRD peak gave information about the phase purity, size, internal crystalline structure and nature of the synthesized AgNPs. It was suggested that AgNPs synthesized from the extract of Aspergillus fumigatus could be a great importance in the pharmaceutical and medical fields. While, the combination of AgNPs and crude extract Aspergillus fumigatus enhances their antimicrobial effect which increase their importance in future studies